Diagnostic Efficiency of Heat Shocked Protoscoleces Extract Antigens for Human Cystic Echinococcosis by ELISA

Diagnosis of cystic echinococcosis is complex and has to be confirmed by the combination immunological tests imaging techniques. In this study heat shock proteins were induced their immunoreactivity was assessed ELISA. Sera collected from 34 hydatid patients who admitted Rizgary Teaching Hospital through October 2013 July 2017, in addition 29 healthy donors 18 non-hydatid cases. For response, two batches 25000 protoscoleces (Ps) incubated separately at 42°C 45 °C for 4 hours. Heat treated normal Ps disrupted extracts divided into parts. One part directly used as source antigens (PE, PE42 PE45) other one partially purified on Sephadex G150. The these antigens, well fluidwas cutoff value differentiate positive negative sera established receiver operating characteristic (ROC) analysis. Two peaks three PE45 resulted Extracts 42ºC protein PE42P1 PE42P2 antigens. 45°C Ps, chromatography patterns PE45P1, PE45P2 PE45P3 Highest rates sensitivity, specificity diagnostic accuracy detected with (91.2%) (91.2%). Sensitivity ELISA consistent liver cysts all applied Hydatid extracted markedly raised sensitivity detect anti-hydatid IgG.